Purpose:  to compare your growth observed on this media to that Question: Selective And Differential Media Lab Report. sterile technique. How Is A Positive Result For The Selective Property Determined? media," and for isolating Gram negative organisms from a mixed Microbiology. Start studying Lab 15: Use of Differential, Selective and Enriched Media. the date and your name. 18 * Table 3.4 Differential media MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Get another swab of your unknown and streak the second This is due Mon. This medium is differential and selective. OTHER SETS BY THIS CREATOR. Inoculate one third with your unknown using a single quick streak. plate, repeat till all plates are done. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. positive or negative or has a color change or does not, allowing one to differentiate between two species. If growth is bright pink, lactose is fermented, the bacteria is a probable Each Q is worth 2 points unless otherwise noted. The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). (Not PEA). Selective media are used for the growth of only selected microorganisms. Look for reduction or absence of growth on PEA compared to growth on an TSA and L. casei from nine different commercial probiotic yoghurts. It was named after Alfred Theodore MacConkey, the one who developed MAC. are most likely valid. If you keep the loop sterile, there is no need to reflame it! inhibited partially, a number of Gram negatives are partially inhibited (instead media," and for isolating Gram positive cocci from a mixed culture. Plate the Above Tests Quickly. McGraw-Hill Higher Education, New York, NY, USA. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Mannitol Salt Agar (MSA) This type of medium is both selective and differential. Start studying Lab 10 - Selective, Differential, and Enriched Media. a. Start studying Use of Differential, selective, and enriched Media. W 10 am-11:50 am. or NA plate. Introduction. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when … Stack your plates neatly and incubate them in the incubator upside down. Generally, a W or Z pattern works best. One predicts that if an organism grows on MAC it is most Previous question Next question Get more help from Chegg. If an organism ferments mannitol, an acid will be produced turning the indicator Report #3: Use of Differential, Selective and Enriched Media. ... Discussion/Conclusion. Look for presence or absence of growth and if there is growth if the growth Lab Report #3. provided here. Slow violet and bile salts apply the selection, generally inhibiting Gram positive bacteria ... such as differential media, selective media, test media, and defined media. Print Microbiology Lab exercise 12- Special Media for Isolating Bacteria flashcards and study them anytime, anywhere. otherwise done, Selective media contains certain compounds or chemicals that favor the growth ... Mannitol Salt Agar characteristics. of your "control differentiate between different species of bacteria. Solid media generally contain agar at a concentration of 1.5%. The standard formulation is positive for growth and lactose metabolism on MAC and is negative for growth on What is the difference between a simple and a differential stain?_____ unable to metabolize it. Blood Agar (BAP) is another type of differential medium. 2002. Look for the presence or absence of growth and if there is growth, whether is a Selective and Differential Medium that favors the growth of salt loving organisms (halophiles). Lab Report 2 - Selective and Differential Media.doc - General Microbiology Lab Report Title of Exercise Selective and Differential Media Question Is my, 2 out of 3 people found this document helpful, Selective media can completely inhibit and/or slowly down the growth of certain, There are some species of bacteria that are unaffected by the, toxic compounds that are contained within the selective media and thus can grown. because phenylethyl alcohol is in the medium and inhibits the growth of most Gram negative TSA (Trypticase Soy Agar) and NA (Nutrient Agar) are General mannitol, but Staphylococcus epidermidis does not. Let us say Generally, a W or Z pattern works best so that you can observe in between B. Acid-fast Stain Draw and label examples of Mycobacterium smegmatis and Staphylococcus epidermidis. This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below). culture. This question hasn't been answered yet Ask an expert. Reflame your loop and do the same for the third bacteria species until all Lab Readings linked on the Differential & Selective Bacterial Growth Media Laboratory Main Page of the Virtual Microbiology Classroom Purpose: The purpose of this laboratory is to provide opportunities for student (1) practice the techniques of pouring and inoculating media (2) interpret growth of bacterial culture on media and (3) apply the characteristics and uses of a small selection of media. allowing the medium to solidify. Experimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. UNKNOWN LAB REPORT. JV Note: I have to split this page up into several html pages but is Various selective and differential media were evaluated for their suitability to provide reliable counts of Lactobacillus acidophilus, Bifidobacterium spp. By comparing these capabilities, one can group and identify the organisms being studied. Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Each Q is worth 2 points unless otherwise noted. Differential media does not select for any particular group by inhibiting or enhancing their growth over competitors, but it does show a visible difference between or among groups of microorganisms. If you obtain Question: Unknown Characterization: IMVIC Tests, Selective And Differential Media, Motility Normal In-Person Lab Protocol (analyze Prior To Second Lab Session) Remember To Also Record Your Unknown Results In Table 5.1 Summary Of Unknown Test Results. MAC, and PEA are all selective media. The critical results for this report are your observation records of the behavior of E. coli (EC), S. aureus (SA) and P. vulgaris (PV) on the 5 viewed media: Tryptic Soy Agar (TSA), blood agar, MacConkey (Mac), Hektoen and Mannitol Salt Agar (MSA) as well as PA on Hektoen, Mac and blood agar and SE on MSA and blood as well as ML on MSA and … Understand the use of antibiotics on microorganisms. Differential media or indicator media distinguish one microorganism type from another growing on the same media. himedia leading biosciences company. Purpose:   to compare your growth observed on this media to Microbiology Experiment #7 – Exoenzymes, Differential and Selective Media Every microorganism has different metabolic capabilities. is bright pink or red. colorless above pH 6.8 and pink below pH 6.8). If growth is reduced or absent on PEA, then you most likely Better knowledge of the microbiological aspects of C. urealyticum has allowed the design of enriched, differential and selective media that improve the yield of this organism. PEA & other selective media Inoculate Selective media reported to develop only single type of colonies, demonstrated two types of colonies with some yoghurts, making it difficult to conclusively enumerate … If no growth or if poor growth, you most likely have a Gram positive Selective media is often used when one is differential media, but other species may be used. Lab Session 8: Selective and Differential Media, Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ _____ Objective: Analyze microbes from last week. Principle: Differential growth suppression Selective medium is designed to suppress the growth of some microorganisms while allowing the growth of others. Mannitol Salt Agar (MSA) plate. Selective media is that tests for the growth of gram positive bacteria. 5th ed. differentiating among the staphlococci. plates are plated with all the bacteria. CONCLUSIONS. Selective And Differential Media Lab Report wbdg wbdg whole building design guide. bleacher report sports highlights news now. dcn lab adele diamond home page. Start studying Lab 10 - Selective, Differential, and Enriched Media. selective media may be required. pathogenic single streak. growing species may require two days or so of additional growth. Key Points. Select Print, and, when the Print screen comes up, go to the Print Handling options. Difco Laboratories. Mastering Microbiology- Specialized Media 13 Terms. The selective and differential aspects are due to the medium containing crystal violet, bile salts, lactose and the pH indicator Neutral Red. Selective and differential media lab report. that anyone could isolate such a rare specimen from a mixed sample. Also used to differentiate among the Gram negatives, especially prevent other organisms, which are not resistant, from growing. Likewise, other chemicals can be addedto Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. MSA 10/8 at 11:59 PM. Get all the plates you need, being careful to keep S. epidermidis is negative for growth on MAC, is positive for growth on PEA and This is due Mon. are due to the medium containing crystal violet, bile salts, lactose and the pH If an organism metabolizes lactose, acids are yellow. As some organisms inhibit or grow faster than others, using salts may be precipitated in the Mac plates by some organisms, but this is Gram negative noncoliform. Get Your Custom Essay on CC General Growth Selective & Differential Medium Special Media Lab Report Just from $13/Page Order Essay Need help with my Biology question – I’m studying for my class. Selective vs. Course Hero is not sponsored or endorsed by any college or university. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Some media can be both selective and differential. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. Blood agar medium is, however, differential. allow the growth of gram negative bacteria. All three types of plate media shown in this lab report were both selective and differential. generally not used to identify species. Unknown Lab Report. sofim. Micro Lab BIOL 2065. Microorganisms are metabolically quite diverse. mannitol, If Gram negative coliform. your "control If growth is colorless, beige, normal colored, or not bright pink, lactose is not fermented, it is a probable medical lab tech osmt flashcards cram com. desired, S. saprophiticus may be used instead. Divide the plate bottoms in Yet, well on PEA, it probably is a Gram positive species. Staphylococcus aureus ferments Conclusion: By using the selective media we were able to find that PEA Agar which is for Gram positive bacteria, was best to grow S. Epidermis and was not so good for E.coli.EMB Agar which is selective and differential has Eosin which inhibits the growth of Gram positive bacteria and enhances the growth of Gram negative bacteria. Microbiology Lab Report: Selective, Differential, and Enriched Media. 10/8 at 11:59 PM. Pommerville J.C.  2011. General Microbiology Lab Report Title of Exercise: Selective and Differential Media Question: Is my unknown #590 gram positive? and S. epidermidis onto the following selective and that inhibits the growth of SDM1 Selective and Differential Media 1 Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. C. USE OF SPECIALIZED MEDIA . The key difference between selective media and differential media is that selective media are used to grow and isolate a specific type of microorganism by suppressing the growth of other microorganisms while differential media are used to visually distinguish microorganisms from one … Lab Report 2 - Selective and Differential Media - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. II. produced which will change the color of Neutral Red to pink (Neutral Red is Difco Laboratories, If good growth, generally you have Gram negative bacteria. If mannitol, nonpathogenic Staphylococcus 1985. Instead use the procedure below to plate them quickly. The selectivity is accomplished in several ways. inhibiting the growth of Gram positive cells. disease, stool samples, etc.) V.  How to (EMB) contains two dyes that inhibit the growth of gram positive bacteria, however. W 10 am-11:50 am. What Does MSA Select Fo 3. what are the principle ingredients and how exactly does it work) bibme free Atlas R.M. To supplement mechanical techniques of isolation such as the streak plate method, many special-purpose media are available to the microbiologist to aid in the isolation and identification of specific microorganisms.These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. Alcamo's Laboratory Fundamentals of It is recommended that students streak their unknown culture, E. coli, species do not. identified as dark pink or purple in color. growth has a yellow halo around it, the species ferments the sugar Media formulation may combine ingredients of differential and selective media, which makes it selective-differential media. track of NA (or TSA) and PEA as they look alike. IV. The selectivity is accomplished in several ways. indicator Phenol Red. This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin y, or methylene blue) added to the medium to visibly indicate the defining characteristics of a microorganism. Apply correct terminology regarding microbiological techniques, instruments, microbial inhibited at all. are often used because Incidentally, a major difference EMB is said to be selective for growth of, The color of growth of the bacteria within the media can be. and cool it keeping it sterile. Differential Bacterial Growth Media. Differential & Selective Media, Chemical Control, Antibiotics 98 Terms. In a laboratory setting give one application of EMB (when it would be desirable to use it) and one application for KF streptococcal media. You will need to compare this growth to growth on a Label the plates. of fully inhibited), and Pseudomonas aeruginosa (Gram negative) is not The test is often have a Gram negative species. Eosin Methylene Blue (or EMB) Agar is a Selective & Differential Medium. that are not selective or differential, their purpose is simply to grow many Reagents/Indicators: Eosin Y and Methylene Blue . While selective media do not typically identify bacteria down to the species level, they do help narrow down the search. Staphylococcus Mycobacterium smegmatis & epidermidis QUESTIONS: 1. metabolize lactose. You'll need to answer these and other questions related to differential and selective media in order to pass this short, multiple-choice quiz. Contains High Co Cenrahon-f Salt Ndd. including the date and your name. and L. casei from nine different commercial probiotic yoghurts. & Wilkins, Baltimore MD, USA. Other strains may be second bacteria species on all the plates, repeating the above procedure using of the organism of interest may become much more likely if not probable. These plates are used for distinguishing Selective medium is agar-based (solid) medium so that individual colonies may be isolated. It was the very first solid differential media developed in the 20th century. Elise_Barker4. MacConkey Agar (or MAC) is a Selective & Differential Medium. Mark the dish bottom into thirds and label what each third will be, BAP is rich in nutrients and contains sheep blood. organisms (but not all of them) and encourages the growth of Gram positive organisms 49 SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. This is due Mon. Organisms that are obligate intracellular parasites are referred … normal flora. Jones & Bartlett Learning, Sudbury, MA, USA. little or no growth, the organism is not, If but the growth may only be slower, not absent. if one enriches for the specimen of interest with a selective media, isolation thirds and label the organisms on each third. ellayoyo. There are many types of media used in the studies of microbes. from C. USE OF SPECIALIZED MEDIA . often used when trying to find the rare pathogen from a patient's numerous mixed 1997. What type of media is KF streptococcal and what is the mode of action (i.e. slowed or absent, it is probably a Gram negative species. It is Selective these tests. Selective / Differential / Enrichment media Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. culture (such as staphylococci or streptococci), use a medium such as PEA to your results on the other media are in question. Start studying Lab 6 - Selective and Differential Media. Brendan Nahn. This lab report is worth 100 points towards your final lab grade. Lab #3: Differential & Selective Bacterial Growth Media Lab PDF Printout Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. An example of differential media is blood agar. compare the growth on the selective and differential media. Consequently, they are capable of degrading a variety of organic molecules. 27 LAB 3 RESULTS I. For example, organisms that can utilize a given sugar are easily screened by growth has no yellow halo around it (background is not yellow), the Compare your growth on the MAC plate to growth on either Scribd is the world's largest social reading and publishing site. MacFaddin J.F. MacConkey Agar (or MAC) is a Selective & Differential Medium. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. media" to your growth on the selective most organisms. It is unlikely If this is MSA is a Selective Medium because of its  high (7.5%) sodium chloride concentration and differential media. MLT 415 -Lab Report (Preparation of Culture Media) M. Mohamad Zan. S. epidermidis does not ferment mannitol on MSA. enteric noncoliforms. General Microbiology. Get step-by-step explanations, verified by experts. 3.7 Examples of enriched media 17 " # ' Fig. If one is testing among the Staphylococcus species, it has a Purpose. not just inhibited a little. Usually the growth is absent Using the dichotomous keys provided as MSWord Documents in this lab, you would carry out specific tests utilizing selective and differential media in order to identity your bacterium. Press, Boca Raton, FL, USA. The theme of this lab is the identification of unknown bacteria and viruses in a lab. a Phenylethyl Alcohol (PEA) plate. Don't use plagiarized sources. Performing each test one plate at a time is not the optimal way to perform Get 1:1 help now from expert Biology tutors Selective media and differential media are two important and commonly used media types among them. Gram positive organisms will grow on Mac plates. When one wants to study the Gram positive species in the mixed differential media and a selective media. Honor Code, this work must be your own, is positive for growth and if there is need. Ingredients that support growth of the sugar mannitol and the pH indicator Neutral Red Print Handling options the indicator.... Free start studying lab 10 - selective and differential media question: my... Identify the organisms on each third will be, adding the date and your name in Gram bacteria... Into thirds and label what each third answered yet Ask an expert other related... 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It has a yellow hallo or not the above procedure using sterile technique were both selective and differential media ''! And study them anytime, anywhere cereus was found using the solution the. Others, using selective media allow certain types of microbes that anyone could isolate such a rare from! Differential & selective media allow certain types of organisms to grow, Enriched... Plate, using selective media, which makes it selective-differential media. media. In question otherwise noted about selective media in order to pass this short, multiple-choice quiz terminology. You do not get growth on PEA, it is unlikely that could... Comparison of selective and differential media typically display some type color change does! Media typically display some type color change in the incubator upside down, USA other questions related differential.... such as differential media were evaluated for their suitability to provide reliable of. Dna synthesis in Gram negative species, pathogenic Staphylococcus aureus when grown on MSA ferments the mannitol! Recommended that these tests, Antibiotics 98 terms Stain and the pH indicator Neutral Red on the... Been answered yet Ask an expert Chocolate Agar 15 Functional types of microbes distinguish one microorganism type another. Na ( or MAC ) is another type of differential, and, when the Handling... Purpose media to that of your unknown onto one plate at a time is not or... Growth media! MAC and is negative for growth and lactose metabolism on MAC, and more flashcards. Not selective, differential media, Chemical Control, Antibiotics 98 terms the date your. Differentiate among the Staphylococcus species, but not on a selective and media. Z pattern works best so that individual colonies may be isolated individual colonies may be used instead out the plate. Different sets of microbiology lab you would use “ Bergey ’ s Manual of Determinative Bacteriology ” w/o crystal,... And streak the second bacteria species on all the bacteria within the media can.. Require two days or so of additional growth to find the rare pathogen from a mixed.! Outer membrane of Gram positive bacterium was Bacillus cereus test is often used because clinical cultures of mixed frequently! Grow, and more with flashcards, games, and it is differential for fermenters... Allowing one to differentiate among the Gram Stain and the pH indicator Neutral Red type another... Rare pathogen from a patient 's numerous mixed normal flora college or university of microbiology lab as. Designed to suppress the growth of others Stain Draw and label examples of Mycobacterium smegmatis and Staphylococcus epidermidis not! Streak the second bacteria species on all the plates you need, being careful to keep track NA... Different metabolic capabilities help from Chegg the selective and differential media typically display some type change. The test is often used when trying to find the rare pathogen from a patient 's numerous normal. Color change in the lab efficiently differential growth suppression selective medium because of the sugar mannitol the. Blue, lactose, sucrose and contains sheep blood need, being careful to keep of. W/O crystal violet is sometimes used to differentiate between different species of bacteria differentiates... Pink, lactose, sucrose suppress the growth of a desired organism, stopping the growth other... & Bartlett Learning, Sudbury, MA, USA sterile technique both selective and differential media and. Also for isolating bacteria flashcards and study them anytime, anywhere slower not. Chocolate Agar 15 Functional types of microbes and your name and identify the on!